The Effect of n-Butanol Extraction of Potentilla anserina L. on Hypoxia-Induced Inflammatory Factors Expression of Wistar Rat Cortical Neuron

The study aims to investigate the effects of n-butanol extraction of Potentilla anserina L. (NP) on cortical neuronal cultures and subject to 2h of oxygen deprivation hypoxia-like injury. The investigation demonstrated that NP at 2, 1 and 0.5 mg/ml effectively prevented the decrease of neuron survival rates and the increase of LDH release after exposure of cortical cultures to 2h-oxygen deprivation. These results indicated that NP has neuroprotective effect against neuronal cell death, induced by oxygen deprivation. High-throughout suspension array technology for simultaneous detection of multiple inflammatory factors expression showed that IL-1β, IL-6 and TNF-α participated in the neuroprotective effect against oxygen deprivation-induced neuronal death.